This course integrates the disciplines of cellular biology, molecular biology, and protein chemistry through a series of related experiments. The course will expose students to: 1) recent journal articles within the scientific literature; 2) selected methods, techniques, and instruments used in biotechnology; and 3) strategies that can be employed to solve interesting biological problems. The laboratory experience will introduce the student to DNA amplification by the polymerase chain reaction; oligonucleotide-directed site specific mutagenesis; gel electrophoresis; isolation of protein, DNA and RNA; gene cloning; DNA sequencing; cell culture; gene expression in mammalian cell lines; and Southern hybridization analysis. Class meets six hours per week for lecture, laboratory, and discussion.
Prerequisites
CHE 202 and BIO 340.
Semester Offered
Spring
Lab Fee
$485